Primer walking is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases.
Primer
A short oligonucleotide complementary to target DNA and acts as the leader for DNA extension. Also refers to short oligonucleotide used to primer reverse transcription of RNA to DNA in reverse transcription reactions. See PCR Glossary.
Primer on Molecular Genetics
from the U.S. Department of Energy
This primer was prepared by Denise Casey, Human Genome Management Information System, Oak Ridge National Laboratory, for the 1991-92 DOE Human Genome Program Report.
Primer
This article is about molecular biology for alternate uses, see primer (disambiguation).
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Definition of primer :
A short sequence (of RNA or DNA) from which DNA replication can initiate. May be either a synthetic DNA or RNA or a length of RNA synthesized in vivo by primase.
Primer
A primer is a short, single-stranded DNA sequence used in the polymerase chain reaction (PCR) technique. In the PCR method, a pair of primers is used to hybridize with the sample DNA and define the region of the DNA that will be amplified.
Primer Pheromones
Rats and mice give off pheromones that elicit mating behavior. However, the response is not immediate as it is in the releaser pheromones of mother rabbits and insects.
Primer
A short oligonucleotide complementary to a strand of DNA or RNA that is used to initiate synthesis of the complementary DNA strand.
primer
An already existing short RNA chain bound to template DNA to which DNA nucleotides are added during DNA synthesis.
primate ...
Primer. A short DNA or RNA fragment annealed to single-stranded DNA, from which DNA polymerase extends a new DNA strand to produce a duplex molecule.
Primer In the elongation of polymers, the initial segment of the polymer that is to be extended; elongation depends on the primer.
Primer: Genomics and Its Impact on Science and Society: The Human Genome Project and Beyond (2008) -- covers basic science, the Human Genome Project, what we know so far, ELSI, medicine, benefits and more.
Primer
Short preexisting single-stranded polynucleotide chain to which new deoxyribonucleotides can be added by DNA polymerase. It anneals to a nucleic acid template and promotes copying of the template starting from the primer site.
Related Terms: ...
Primers constructed from the flanking regions of microsatellite DNA which can be used in PCR reactions to amplify the repeat region.
Related Terms:
Primer ...
Primer
- A substance that is required for a polymerization reaction (e.g., DNA synthesis) and is structurally similar to the product of the reaction itself
Prokaryotes ...
Primers are short chains of about 20 nucleotides that are complimentary to a region in the DNA to be amplified. They are needed because the enzyme that copies the DNA (DNA polymerase) cannot start the process unless it has already been started.
PRIMER - An oligonucleotide which is complementary to a specific region within a DNA or RNA molecule, and which is used to prime (initiate) synthesis of a new strand of complementary DNA at that specific site, ...
Primer extension: This is a method used to figure out how far upstream from a fixed site the start of an mRNA is. For example, perhaps you have isolated a cDNA clone, but you don't think that the clone has all of the 5' untranslated region.
RNA primers - primers used to synthesize DNA strands by acting as a template
RNA splicing - process in which intron sequences are excised from RNA molecules in the nucleus during formation of messenger RNA ...
Problem 1 PCR primers
Which of the following primers would allow copying of the single-stranded DNA sequence 5' ATGCCTAGGTC?
A. ...
Most often used to describe the binding of a short primer or probe. Antibiotic. A class of natural and synthetic compounds that inhibit the growth of or kill other microorganisms.
Polymerase chain reaction (PCR) A method for amplifying a DNA base sequence using a heat-stable polymerase and two 20-base primers, ...
In practice, when synthesising an oligonucleotide primer or probe based on this information a mixture of all the possible sequences is made, and referred to as a degenerate oligonucleotide.
As it cools, primers bind the DNA. These primers are short oligonucleotides chosen by the experimenter and added to the DNA mixture at the beginning. They flank the region to be amplified.
For the primers to anneal to the ssDNA fragments, the temperature is reduced to about 55° C. However, at this temperature the original complementary ssDNA fragments will begin to re-anneal with each other.
For instance replication requires a primer and specificity is determined by the sequence and size of the primer.
Evolution and the Fossil Record This primer from the American Geological Institute is available by following links from this page to an ADOBE PDF file that can be viewed and printed from your computer.
It contains the sites where the first separation of the complementary strands occurs, a primer rNA is synthesised, and the switch from primer rna to dNA synthesis takes place. (rieger et al., glossary of genetics: classical and molecular, 5th ed) ...
In this method, genomic DNA is amplified by PCR using specific sets of primers complementary to a given region. The PCR product is then digested with a restriction enzyme and the products separated on an agarose gel.
Source: The Brain: A Neuroscience Primer written by Richard F. Thompson, 3rd edition (User Submission)
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Primer on Molecular Genetics (Department of Energy)
Tutorial on EUKARYOTIC DNA TRANSCRIPTION (UC Davis)
Glossary (DOE) Terms peculiar to molecular genetics.
Gene Zine A very good beginning on how genes work.
Enzymes producing a polynucleotide sequence complementary to a pre-existing template polynucleotide. DNA polymerase requires a primer from which to start polymerisation whereas RNA polymerase does not ...
DNA polymerase an enzyme which makes DNA from nucleotide triphosphates. Usually it requires a single stranded template (either DNA or RNA) and a short primer (again either DNA or RNA) to enable it to make a complementary copy.
Primer -- nucleotides used in the polymerase chain reaction to initiate DNA synthesis at a particular location. Probability -- the long term frequency of an event relative to all alternative events, and usually expressed as decimal fraction.
 See also: DNA, Sequence, PCR, Biology, Trans
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