probe
a defined segment of DNA or RNA, usually carrying a radioactive label, used to identify a specific segment of DNA that carries the complementary base sequence ...
probe
(Science: molecular biology) general term for a piece of dNA or rNA corresponding to a gene or sequence of interest, that has been labelled either radioactively or with some other detectable molecule, such as biotin, digoxygenin or fluorescein.
The increase in the amount of probe molecules specific to a given target so that they can be easily detected. Examples include Q-beta and ligase chain reaction.
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...
Probe
A probe is a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a sample genome.
Probe hybridization. Joe Gray [University of California, San Francisco (UCSF) and LBL] reported on the LBL-UCSF Resource for Molecular Cytogenetics, ...
Probe mapping and ordering: High resolution mapping and ordering of DNA molecules is required for tasks such as contig assembly.
Probe A labelled molecule which will recognise and bind to a specific target molecule, thus allowing detection of the target. DNA probes are used to locate and quantitate DNA (or RNA) of complementary sequence.
Probe
A fragment of DNA labeled with radioactivity or chemiluminescence and used to hybridize to another DNA molecule to identify complementary base sequences.
Probe A DNA or RNA molecule which has been labelled and which may be used to identify its complementary sequence by hybridisation.
Probe. A single-stranded DNA that has been radioactively labeled and is used to identify complementary sequences in genes or DNA fragments of interest. (See Multilocus probe.) ...
DNA probe
A chemically synthesized, radioactively labeled segment of nucleic acid used to find a gene of interest by hydrogen-bonding to a complementary sequence.
domain ...
DNA probe A radioactively labeled, single-stranded specific base sequence used to locate a complementary sequence among DNA fragments displayed on an electrophoretic gel.
Re-probe southern blot with additional probes
In a typical forensics DNA analysis, DNA polymorphisms on several different chromosomes are characterized.
Probes can be synthesized using a DNA synthesis machine. This requires a knowledge of at least a part of the sequence of the nucleotides in the gene to be probed so that a complimentary fragment can be created.
Probe: A fragment of DNA or RNA which is labeled in some way (often incorporating 32P or 35S), and which is used to hybridize with the nucleic acid in which you are interested.
PROBE - Usually refers to a DNA or RNA molecule which has been labeled with 32P or with biotin, to facilitate its detection after it has specifically hybridized with a target DNA or RNA sequence.
If probes encounter a complementary sequence of nucleotides in a test sample of DNA, they bind to it by Watson-Crick base pairing and thus identify it.
Polymorphisms are inherited differences found among the individuals in a population.
DNA probes
See probe.
Related Terms:
Probe
Single-stranded DNA or RNA molecules of specific base sequence, labeled either radioactively, immunologically, or by other means, that are used to detect the complementary base sequence by hybridization.
DNA probe
- A molecule that has been labeled with a radioactive isotope, dye or enzyme and is used to locate a particular portion of a DNA molecule.
DNA sequence ...
NANOPROBE SCANNING
A family of imaging techniques in which the interaction of a sharp, nanometre-sized tip with a specimen is measured as the tip is mechanically scanned over the specimen surface.
OPTICAL COHERENCE TOMOGRAPHY ...
Fluorescent probes can be attached to specific subcellular components, such as the chromosomes and microtubules, for visualization of mitosis using standard epi-fluorescence microscopy techniques.
In scanning probe microscopy (SPM), a physical probe is used either in close contact to the sample or nearly touching it.
labelling a probe with congugated biotin, whose high affinity for avidin or anti-biotin antibody is exploited in immunoassays ...
DNA probe -- any biochemical used to identify or isolate a gene, a gene product, or a protein.
DNA probes See probe.
DNA replication The use of existing DNA as a template for the synthesis of new DNA strands. In humans and other eukaryotes, replication occurs in the cell nucleus.
Most often used to describe the binding of a short primer or probe. Antibiotic. A class of natural and synthetic compounds that inhibit the growth of or kill other microorganisms.
So it becomes really valuable to go in and actually look at tissues directly, at sections of tissues, and probe them with different probes that pick up different kinds of sugars. This, in a general sense, is glycobiology and it is very valuable.
Signal Quantitative metric calculated for each probe set using Affymetrix software, which represents the relative level of expression of a transcript.
The probe hybridized in normal hemoglobin with two fragments 7000 or 7600 nucleotides long. Sickle-cell hemoglobin had hybridization with a 13,000 nucleotide single sequence.
Studies to probe the regulation of μ2 phosphorylation demonstrated that clathrin is a specific activator of the μ2 kinase, and, in permeabilized cells, cargo sequestration, driven by exogenously added clathrin, ...
Use of a DNA or RNA probe to detect the presence of the complementary DNA sequence in chromosome spreads or in interphase nuclei or an RNA sequence of cloned bacterial or cultured eukaryotic cells.
Earth Probe TOMS ozone hole animation for 1997 View an animated GIF image of the ozone hole over Antarctica for August to November 1997. Other data from the Total Ozone Mapping Spectrometer (TOMS) is also available here.
Research primarily involves studying data from telescopes and planetary space probes, comparing the conditions on other planets in the solar system with extreme environments on Earth.
(fluorescence in situ hybridization): One of the more modern methods in cytogenetics, which uses fluorescence-labelled chromosome-specific DNA, probes to detect translocations, inversions, deletions, ...
The closest linked marker (e.g., EST or a known gene) to the gene is used to probe a genomic library. A restriction fragment isolated from the end of the positive clones is used to reprobe the genomic library for overlapping clones.
A number of fluorescent probes have been developed that can be used to label specific organelles, surface structures, live versus dead bacteria (below, left) and even ions that move into and out of the cells.
scanning tunneling microscope The type of microscope that uses a needle probe and electrons to determine the surface features of specimens.
scaphoid scaph = a boat or a bowl.
trier - small metal probe for taking samples of paddy or rice or from bulk containers (Glossary of PM)
triungulin - the earliest larval stage of some beetle species ...
Sets of miniaturized chemical reaction areas that may also be used to test DNA fragments, antibodies, or proteins, by using a chip having immobilised target and hybridising them with probed sample.
Probelesodon has developed a double joint in the jaw. The jaw could hinge off two points with the upper skull. The front hinge was probably the actual hinge while the rear hinge was an alignment guide.
The labelled probes are allowed to bind to complementary DNA strands on the slides.
566- Superior sagittal sinus laid open after remova of the skull cap. The chordæ Willisii are clearly seen. The venous lacunæ are also well shown; from two of them probes are passed into the superior sagittal sinus.
- coming together (annealling) of single-stranded nucleic acid sequences by hydrogen bonding of complementary bases to form double-stranded molecules; this process is the basis for molecular biological techniques in which a labeled probe sequence is ...
Southern blotting - technique in which DNA fragments, separated by electrophoresis, are immobilized on a paper sheet; specific molecules are then detected with a labeled nucleic acid probe ...
 See also: DNA, Human, Sequence, Trans, Genome
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