Reporter Genes
With gene splicing, the promoter of a gene whose expression you wish to monitor can be coupled to the coding sequence of a chosen "reporter" gene.
lacZ
One favorite reporter gene is the Z gene of the E.
Reporter gene
A gene which can be placed downstream of a promoter and expression of the gene followed by a relatively easy assay (often a colorimetric assay). See operon and gene fusions.
Reporter gene Gene coding for an easily assayed protein which is used to detect expression of the gene under different conditions; usually to test the activity of a promoter.
Reporter gene See: marker
Resolution Degree of molecular detail on a physical map of DNA, ranging from low to high.
a reporter gene that codes for beta galactosidase.
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In such a construct, a promoter sensitive to enhancer regulation is fused to a reporter gene, such that expression patterns of the reporter gene identify the spatial regulation conferred by nearby enhancers.
It is performed with gene trap vectors whose principal element is a gene trapping cassette consisting of a promoterless reporter gene and/or selectable genetic marker flanked by an upstream 3' splice site (splice acceptor; SA) and a downstream ...
Using genetically engineered yeast, scientists can identify complexes when specific pairs of interacting proteins activate expression of a reporter gene. One often-used reporter gene is the lacZ gene.
gene trap Gene traps, also known as promoter traps or exon traps, are type of DNA construct containing a reporter gene downstream of a splice acceptor site.
AP-1 activation through EGFR was assessed using a β-lactamase reporter gene ... An AP-1 response element (TGACTAA, 7X) was inserted into the MCS of the β ...
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Chloramphenicol acetyl transferase (CAT): The bacterial gene for chloramphenicol, CAT, is commonly used as a reporter gene for investigating physiological gene regulation.
Chloramphenicol Acetyl Transferase (CAT) is a bacterial enzyme which inactivates chloramphenicol by acetylating it. Used as a reporter gene for promoter studies.
Related
GFP Luciferase assay ...
It is easier to perform a CAT assay than it is to do a Northern blot, so CAT assays were a common method for testing the effects of sequence changes on promoter function. Largely supplanted by the reporter gene luciferase.
 See also: Gene, Express, Trans, Organ, Protein
  
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