restriction fragment length polymorphis (RFLP)
a DNA polymorphism detected with a restriction enzyme and a specific DNA probe
Source: Jenkins, John B. 1990. Human Genetics, 2nd Edition. New York: Harper & Row ...
Restriction Fragment Length Polymorphism (RFLP)
Sequence polymorphism which results in changes in the restriction map.
Other Resources ...
Restriction fragment length polymorphism
In molecular biology, the term restriction fragment length polymorphism (or RFLP) is used in two related contexts: as a characteristic of DNA molecules (arising from their differing nucleotide ...
Restriction Fragment Length Polymorphism (RFLP)
Restriction fragment length polymorphism (RFLP) is a type of polymorphism that results from variation in the DNA sequence recognized by restriction enzymes.
restriction fragment length polymorphism (RFLP) A heritable difference in DNA fragment length and fragment number; passed from generation to generation in a codominant way.
Restriction fragment length polymorphism (RFLP)
Variation between individuals in DNA fragment sizes cut by specific restriction enzymes; ...
Restriction fragment length polymorphisms (RFLP) are variation in DNA fragment banding patterns of electrophoresed restriction digests of DNA from different individuals of a species.
Restriction fragment
A DNA fragment which has been cut by a restriction enzyme.
Related Terms:
Deoxyribonucleic acid (DNA) ...
Restriction fragment: The piece of DNA released after restriction digestion of plasmids or genomic DNA. See "Restriction enzyme". One can digest a plasmid and isolate one particular restriction fragment (actually a set of identical fragments).
Using restriction fragment length polymorphism (RFLP) analysis, White and Cavenee demonstrated the first proof that loss of a protective suppressor gene, one that prevents cells from becoming malignant, can trigger cancer [Nature 305: 779-784 (1983)].
Using restriction fragment polymorphisms (RFLPs) to map the location of disease-causing genes in humans.
Genetic versus Physical Maps ...
RFLP (or Restriction fragment length polymorphism)
AFLP (or Amplified fragment length polymorphism)
RAPD (or Random amplification of polymorphic DNA)
VNTR (or Variable number tandem repeat)
Microsatellite polymorphism ...
RFLP Restriction Fragment Length Polymorphisms. These markers detect polymorphic sequences using a labelled probe hybridized against digested genomic DNA.
RFLP
See restriction fragment length polymorphism.
Related Terms:
Restriction fragment length polymorphism (RFLP) ...
DNA insert and vector molecules are digested with two different restriction enzymes to create noncomplementary sticky ends at either end of each restriction fragment.
See RFLP, restriction fragment length polymorphism.
Mb See megabase.
Megabase (Mb) Unit of length for DNA fragments equal to 1 million nucleotides and roughly equal to 1 cM.
Meiosis Cell division to produce sex cells.
RFLPRestriction fragment length polymorphism. A difference in restriction fragment length between individuals due to loss or gain of a restriction enzyme site due to point mutation, or insertion or deletion between consecutive sites.
A more accurate test, which can also be administered earlier during pregnancy, involves the use of a radioactive probe (36 nucleotide RNA fragment) which hybridizes restriction fragments.
What is the difference between a restriction fragment length polymorphism (RFLP) and a single nucleotide polymorphism (SNP)?
Chromosome walking
A technique used to identify a series of overlapping restriction fragments, often to determine the relative positions of genes on large chromosomes.
A procedure in which DNA restriction fragments are transferred from an agarose gel to a nitrocellulose filter, where the denatured DNA is then hybridized to a radioactive probe (blotting). (See Hybridization.) ...
Cut the DNA (cleavage) after enzymes have attached to all recognition sites
Fragments produced are called restriction fragment length polymorphisms (RFLPs)
Some produce blunt ends, some sticky ends (more useful) ...
 See also: Restriction, DNA, Polymorphism, Sequence, Chromosome
  
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