Restriction site
DNA sequence recognized and cut by a restriction enzyme.
Related ...
restriction site
A specific sequence on a DNA strand that is recognized as a "cut site" by a restriction enzyme.
reticular formation ...
Restriction sites Short, often palindromic DNA sequences which are the sites for recognition by restriction endonucleases.
Retroposition The integration of a sequence derived from RNA into a DNA genome. See retrovirus.
Restriction site
Shortened term for restriction endonuclease recognition sequence.
Related Terms:
Restriction enzyme
A bacterially derived protein that recognizes specific, short nucleotide sequences and cuts DNA at those sites.
RESTRICTION SITE - See RECOGNITION SEQUENCE.
RETICULOCYTE LYSATE - A lysate of rabbit reticulocytes, which has been extensively digested with micrococcal nuclease to destroy the reticulocyte mRNAs.
Restriction site: See Restriction enzyme.
Reverse transcriptase: An enzyme which will make a DNA copy of an RNA template - a DNA-dependant RNA polymerase.
"Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.". Science (New York, N.Y.) 230 (4732): 1350-4. doi:10.1126/science.2999980. PMID 2999980.
Because large fragments of randomly sheared DNA are mapped with high cutting efficiency, many overlapping restriction site landmarks allow contigs to be assembled and a shotgun mapping strategy can be employed.
So basically, if you follow the sequence of DNA, particular sites, a series of four to eight nucleic acids, results in a restriction site where an enzyme from bacteria can actually bind and cleave that DNA. So why is that useful?
The distance between the locations cut by restriction enzymes (the restriction sites) varies between individuals: so the length of the fragments varies, and the position of certain gel bands differs between individuals (thus polymorphism).
- A diagram that shows restriction sites (i.e., where a restriction enzyme cleaves DNA) in relation to one another.
Restriction Fragment Lenght Polymorphism (RFLP) ...
Physical map. A map showing physical locations on a DNA molecule, such as restriction sites, and sequence-tagged sites. (See Mapping.)
Plant Pest Act (PPA). See U.S. Department of Agriculture.
Polymorphic sequences which alter the restriction site (adding, removing a site or changing the size of the digest fragment) are separated on an agarose gel, transferred to a membrane and hybridized to a cloned piece of DNA.
Insertional inactivation Gene inactivation that occurs when an unrelated DNA fragment is inserted at a restriction site; ...
Radioactive probes (32p) will give off radiation causing a pattern of bands on the X-ray plate, conforming the presence of the disease causing gene
Mutant gene is missing a restriction site which is present at normal genes ...
MHC-linked 21-hydroxylase gene (CYP21), gene conversions within the MHC class II genes in mice and humans, many oncogene translocations (BCL2 for example) are attributed to chi-like sequences at the breakpoint region. It acts like a restriction site ...
 See also: Restriction, Sequence, DNA, Enzyme, Restriction enzyme
  
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