RT-PCR: (Reverse Transcription PCR) is a method used to amplify, isolate or identify a known sequence from a cellular or tissue RNA. The PCR is preceded by a reaction using reverse transcriptase to convert RNA to cDNA.
RT-PCR See Reverse Transcription-PCR (RT-PCR)
Recombinant
Recombinant DNA (rDNA)
Recombination frequency
Replication fork
Response element
Restriction
Restriction Fragment Length Polymorphism (RFLP)
Restriction enzyme
Restriction map ...
New Gene-Expression Data and Query Form: Data set of RT-PCR assays produced by Tom Freeman's laboratory represents analyses of 517 genes in 46 mouse tissues from animals 6 to 8 weeks old and studies of tissues from 15-day-old mouse embryos.
Differential displayA form of RT-PCR in which primers are used to select a subset of the total mRNA population. This allows comparison of mRNAs from different cells.
Once RNA is isolated it can be reverse transcribed back into DNA (complementary DNA to be precise, known as cDNA), at which point traditional PCR can be applied to amplify the gene, this methodology is called RT-PCR.
quantitative PCR
quantitative RT-PCR
quantitative trait
quantitative trait locus
quantitative variable
quantitative variation
Quantitative-fluorescent polymerase chain reaction
quaternary structure
quiescent ...
May be combined with reverse transcription of mRNA to cDNA to amplify an mRNA so called RT-PCR.
Related Terms:
Base sequence
The order of nucleotide bases in a DNA molecule. Length is usually defined as the number of base pairs. Cf.
Several variations have been developed for specific needs. May be combined with reverse transcription of mRNA to cDNA to amplify an mRNA so called RT-PCR.
Arbitrarily primed polymerase chain reaction (AP-PCR) ...
(copy) DNA (cDNA): Single-stranded DNA produced from an RNA template (usually mRNA) by reverse transcriptase in vitro. It lacks the introns present in corresponding genomic DNA. It is most commonly made to use in PCR to amplify RNA (RT-PCR).
even if mixed in with other undesirable DNA, can be amplified to obtain billions of replicates. PCR can be used to amplify RNA sequences if they are first converted to DNA via reverse transcriptase. This two-phase procedure is known as ‘RT-PCR'.
 See also: PCR, Trans, DNA, Sequence, Transcription
  
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