S1 nuclease

S1 NUCLEASE - An enzyme which digests single-stranded DNA or RNA
SDS-PAGE - Denaturing protein gel electrophoresis (see POLYACRYLAMIDE GEL ELECTROPHORESIS).

S1 nuclease: An enzyme which digests only single-stranded nucleic acids.
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The resulting single-strand gaps are converted to double-strand gaps by treatment with S1 nuclease, and the ends are repaired and ligated with T4 DNA polymerase and ligase.

In endonuclease, it cleaves at internal sites in the substrate molecule. Exonuclease progressively cleaves from the end of the substrate molecule. In ribonuclease (RNase), the substrate is RNA. In the S1 nuclease, ...

In the S1 nuclease, the substrate is single-stranded DNA or RNA. Nucleic acids. The two nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), are made up of long chains of molecules called nucleotides. See DNA, RNA, Nucleotides.

See also: Species, Nucleotide, PCR, Origin, Polymerase