Shotgun cloning
A cloning strategy that involves the insertion of random fragments of a large DNA molecule into a vector, resulting in a large number of different recombinant DNA molecules.
SHOTGUN CLONING or SEQUENCING - Cloning of an entire genome or large piece of DNA in the form of randomly generated small fragments. The individual sequences obtained from the clones will be used to construct contigs.
Shotgun cloning: The practice of randomly clipping a larger DNA fragment into various smaller pieces, cloning everything, and then studying the resulting individual clones to figure out what happened.
The sequencing of complex genomes requires shotgun cloning (or subcloning) of genomic DNA (or BACs) into vectors that carry smaller inserts and that can serve as templates in sequencing reactions.
 See also: Insert, Genome, Vector, DNA sequence, DNA
  
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