Shotgun sequencing

Shotgun Sequencing
Shotgun sequencing is a laboratory technique for determining the DNA sequence of an organism's genome. The method involves breaking the genome into a collection of small DNA fragments that are sequenced individually.

Shotgun Sequencing of a Cosmid Using the M13-102 Direct Selection Vector
Richard A. Guilfoyle, Danhua Chen, Arthur Johnson, Todd Francisco, Tetsuyoshi Ono, David Rank, and Lloyd M. Smith ...

Shotgun sequencing - an alternative method, using random, rather than consecutive, sub-strands.
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See more about: Chromosome walking ...

Shotgun sequencing: A way of determining the sequence of a large DNA fragment which requires little brainpower but lots of late nights.

Whole genome shotgun sequencing skips the mapping step of clone-based sequencing.

Three years later, in 1998, the announcement by the newly-formed Celera Genomics that it would scale up the shotgun sequencing method to the human genome was greeted with skepticism in some circles.

Refers to longer fragments of DNA built from short fragments, e.g., a long contiguous DNA sequence assembled from shotgun sequencing.
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Shotgun sequencing ...

A widely used method of determining the order of bases in DNA.
See also: sequencing, shotgun sequencing
Satellite
A chromosomal segment that branches off from the rest of the chromosome but is still connected by a thin filament or stalk.

See also: Sequencing, Sequence, Genome, Human, DNA