Site-specific recombination

Site-specific recombination
Genetic exchange that occurs between particular, short DNA sequences. Site-specific recombination systems do not require RecA.

Site-specific recombination The exchange of two specific but not always homologous DNA sequences.

Main article: Site-specific recombination
In conservative site-specific recombination, a mobile DNA element is inserted into a strand of DNA by means similar to that seen in crossover.

Miles Brennan (NIH National Institute of Mental Health) described using Cre/lox, the site-specific recombination system of bacteriophage P1, to generate transgenic mice with precisely engineered deletions or duplications.

Both int and IHF bind to attP and built an intrasome , a DNA-protein-complex designed for site-specific recombination of the phage and host DNA. The original BOB' secuanes is changed by the integration to B-O-P'-phage DNA-P-O-B'.

Another form of recombination, site-specific recombination, is found where transfer, rearrangement or insertion of specific sections of DNA occurs e.g. insertion of viral DNA into chromosomes, gene switching etc.

See also: Recombination, DNA, Sequence, Cell, Trans