Sticky ends
Digestions of double-stranded DNA by many restriction enzymes (e.g. EcoR I) generate ends with a short single-stranded sequences. Such ends are called sticky ends.
Related ...
Sticky end/blunt end
In biology, sticky end and blunt end are the two possible configurations resulting from the breaking of double-stranded DNA.
Sticky Ends
Fragments of DNA that has been cut with restriction enzymes have unpaired nucleotides at the ends called sticky ends. All of the fragments will have the same sticky ends.
sticky ends Term applied to DNA sequences cut with restriction enzymes where the cuts will bond with each other or with another sequence cut with the same enzyme.
Sticky end. A protruding, single-stranded nucleotide se- quence produced when a restriction endonuclease cleaves off center in its recognition sequence.
STICKY END - The terminus of a DNA molecule which has either a 3' or 5' overhang, and which typically results from a cut by a restriction endonuclease.
Sticky ends: After digestion of a DNA with certain restriction enzymes, the ends left have one strand overhanging the other to form a short (typically 4 nt) single-stranded segment.
These are called "sticky ends" because they are able to form base pairs with any DNA molecule that contains the complementary sticky end. Any other source of DNA treated with the same enzyme will produce such molecules.
(Redirected from Sticky end)
Jump to: navigation, search
DNA end or sticky end refers to the properties of the end of a molecule of DNA.
DNA insert and vector molecules are digested with two different restriction enzymes to create noncomplementary sticky ends at either end of each restriction fragment.
Sticky endShort section of single-stranded DNA produced at the end of a double-stranded DNA fragment produced by DNA digestion with certain restriction enzymes.
This produces either "sticky ends" or "blunt ends"
DNA ligase can be used to re-join the ends ...
DNA ligase - enzyme that cuts DNA, creating sticky ends.
C.
DNA polymerase - copies DNA sequences in the polymerase chain reaction.
Cohesive end: Also known as sticky end. Overhanging ends of a double-stranded DNA molecule that are capable of hybridizing with complementary ends.
Linker
A synthetic, double-stranded oligonucleotide used to attach sticky ends to a blunt-ended molecule.
Some restriction enzymes generate blunt ends, cutting across both strands of DNA. Others generate a staggered cut, producing "sticky ends.
 See also: DNA, Enzyme, Strand, Sequence, Restriction
  
|
RSS
