Stringency
Hybridization conditions: higher the stringency, lower the probability of hybridization. Increase the temperature or decreasing the salt concentration raises the stringency.
Related ...
stringency
(Science: molecular biology) reaction conditions, notably temperature, salt, and pH that dictate the annealing of single-stranded DNA/DNA, DNA/ rNA, and RNA/RNA hybrids.
Stringency
Conditions affecting the hybridization of nucleotide sequences. Higher stringency conditions require more base pairing between the two sequences.
STRINGENCY - The conditions employed for hybridization which determine the specificity of the annealing reaction between two single-stranded nucleic acid molecules.
Stringency: A term used to describe the conditions of hybridization. By varying the conditions (especially salt concentration and temperature) a given probe sequence may be allowed to hybridize only with its exact complement (high stringency), ...
These issues include relative quantitation by low-stringency PCR, the Cot effect, and problems of target vs standard titration.
 See also: Hybrid, DNA, Hybridization, Sequence, Genome
  
|
RSS
