Subcloning
Cloning of the original DNA in a vector to other vectors. The original DNA may be digested to create smaller subclones.
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Subcloning. The process of tranferring a cloned DNA fragment from one vector to another. (See Cloning.) ...
(See Directional cloning, Megabase cloning, Molecular cloning, Subcloning.) Coat protein (capsid). The coating of a protein that enclosed the nucleic acid core of a virus. Codon.
In order to improve upon existing systems, we have developed a new cloning vector that allows double adaptor shotgun subcloning of large target molecules into pUC-18.
DNA end or sticky end refers to the properties of the end of a molecule of DNA. The concept is important in molecular biology, especially in cloning or when subcloning insert DNA into vector DNA.
Chromosome walking A technique for analyzing long stretches of DNA by sequential subcloning and rescreening of overlapping segments.
 See also: DNA, Cloning, Sequencing, Genome, Primer
  
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