Transfection
Methods for delivering DNA/RNA into tissue culture cells, include lipofection, electroporation, and calcium phosphate transfection.
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Definition of transfection :
The process by which exogenous DNA in solution is introduced into cells. The introduction of foreign DNA into eukaryotic or prokaryotic cells.
Transfection
The introduction of purified phage DNA into a bacterial cell by transformation or electroporation.
Transfection: Alteration of the genome of a cell by direct introduction of DNA, a small portion of which becomes covalently associated with the host cell DNA.
transfection - incorporation of DNA into a cell by incubation of the two together under appropriate conditions.
Transfection Transfer of DNA (usually of a gene) into a cultured cell where it can be expressed.
Transgenic animal An animal (nearly always a mouse) into the genome of which a foreign gene has been introduced.
Transfection. The uptake and expression of a foreign DNA sequence by cultured eukaryotic cells.
Transfer DNA. See T-DNA.
Transfer RNA (tRNA). See tRNA.
Transfection
First used for the transformation of prokaryotic cells by protein-free DNA or RNA from viruses. Also the process of genetic transformation in eukaryotic cells.
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Transfection
Triparental mating
[edit] References
^ a b Holmes RK, Jobling MG (1996). Genetics: Conjugation. in: Baron's Medical Microbiology (Baron S et al, eds.), 4th ed., Univ of Texas Medical Branch. ISBN 0-9631172-1-1.
TRANSFECTION - The process of introducing foreign DNA (or RNA) into a host organism, usually a eukaryotic cell.
A gene that upon transfection enables a primary cell to grow indefinitely in culture. (See Oncogene.) Incomplete dominance.
TransfectionA general term to describe the introduction of recombinant or vector DNA into host cells.
bacteriophage, bacteria, virus, plasmid, transfection
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Sometimes viruses pick up a piece of the host cell DNA instead of their own and, in that way, carry genes from one bacterium to another (a process known as transfection).
Furthermore, we describe the development of a reliable transfection method for motor neurons based on microinjection, which will be extremely useful for dissecting further the molecular basis of membrane dynamics and axonal transport in these cells.
 See also: DNA, Trans, Cells, Cell, Protein
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