vectors Self-replicating DNA molecules that can be joined with DNA fragments to form recombinant DNA molecules.
veins Thin-walled vessels that carry blood to the heart. Units of the circulatory system that carry blood to the heart.
Vectors and Biochemistry for Sequencing by Nested Deletions
John J. Dunn and Matthew Randesi
Biology Department, Brookhaven National Laboratory, Upton, New York 11973 ...
Vectors
Vectors are DNA used to transfer genes into a host cell.
A vector must be capable of self-replicating inside a cell.
Vectors in gene therapy
Viruses attack their hosts to insert their genetic material into the genetic material of the host. This genetic material contains instructions to produce these viruses.
[edit] Vectors
Plasmids used in genetic engineering are called vectors. They are used to transfer genes from one organism to another and typically contain a genetic marker conferring a phenotype that can be selected for or against.
Vectors are vehicles for cloning DNA. A vector provides essential sequences for replicating DNA in a host and selection antibiotic markers. Non-essential sequences are deleted to allow room for the cloning of foreign DNA.
Related ...
vectors of human diseases such as
malaria and yellow fever (both transmitted by mosquitoes)
plague (transmitted by fleas)
many crop pests ...
Insect Vectors
Insects provide a system that can deliver pathogens directly to the bloodstream and are essential to the spread of some infections.
phage - virus that infects bacteria; altered phage can be used as cloning vectors. (short for bacteriophage - "bacteria eater") ...
Altered forms are used as vectors for cloning DNA. Bacteriostat. A class of antibiotics that prevents growth of bacterial cells. Bacterium. A single-celled, microscopic prokaryotic organism: a single cell organism without a distinct nucleus.
Cosmids -- plasmid vectors designed for cloning large fragments of eukaryotic DNA; the vector is a plasmid into which phage lambda cohesive end sites have been inserted. CpG islands -- areas of multiple CG repeats in DNA.
coli vectors which are used for cDNA and genomic DNA libraries. Filamentous bacteriophages are used to produce single-stranded DNA for sequencing or in vitro mutagenesis (eg. M13), and as vectors for Phage display expression screening systems ...
Cloning vector DNA molecule originating from a virus, a plasmid, or the cell of a higher organism into which another DNA fragment of appropriate size can be integrated without loss of the vector's capacity for self-replication; vectors introduce ...
So what vectors do is allow you to propagate the DNA you're interested in, in the organism you've chosen to propagate it in.
Cosmids can be packaged in lambda phageparticles for infection into Escherichia coli; this permits cloning of larger DNA fragments (up to 45 kb) than can be introduced into bacterial hosts in plasmid vectors.
Related Terms:
Cloning vector ...
DNA molecule originating from a virus, a plasmid, or the cell of a higher organism into which another DNA fragment of appropriate size can be integrated without loss of the vectors capacity for self-replication; ...
Yeast chromosomes have also been modified to serve as vectors for transporting human DNA fragments for use in gene mapping.
BiBAC Binary bacterial artificial chromosome. BiBAC vectors are used for cloning large DNA fragments (typically 100-200kb) in E.coli. BiBACs can be directly used as a plant transformation vector.
Cosmid
A cloning vector consisting of the phage lambda cos site inserted into a plasmid. Such vectors can be packaged into lambda phage or maintained as plasmids. Cosmids are often used to clone large DNA fragments (up to about 40 kilobases).
pBR322. A derivation of ColE1, one of the first plasmid vectors widely used. (See Plasmid.)
PCR. See Polymerase chain reaction.
Pedigree. A diagram mapping the genetic history of a par- ticular family.
Bacteriophage A virus which infects bacteria. Modified bacteriophages are used as cloning vectors. Two which are commonly encountered are bacteriophage lambda and bacteriophage M13.
MULTICOPY PLASMIDS - Present in bacteria at amounts greater than one per chromosome. Vectors for cloning DNA are usually multicopy; there are sometimes advantages in using a single copy plasmid.
Ticks are vectors of several diseases. The dog tick can carry Rocky Mountain Spotted Fever, caused by Rickettsia rickettsii.
- The agent used to carry new DNA into a cell. Viruses or plasmids are often used as vectors.
Virus ...
Vector: The DNA "vehicle" used to carry experimental DNA and to clone it. The vector provides all sequences essential for replicating the test DNA. Typical vectors include plasmids, cosmids, phages and YACs.
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These molecules bind selectively to the target DNA. Once bound, the chimeraplast activates a naturally occurring gene-correcting mechanism. Does not use viral or other conventional gene-delivery vectors.
man himself does not transport the infestation from place to place: furnishings removed for cleaning are likely to result in infestation. Experiments have shown that bedbugs can transmit a variety of diseases, but they are not normal vectors ...
 See also: Vector, DNA, Sequence, Cells, Trans
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