When the first true leaves appear, thin your "hill" to 2 or 3 plants. Be careful not to disturb roots when you thin the plants. instead, consider cutting off the plants you want to thin at ground level.
When the first true leaves appear, thin the pot or cell to the strongest looking seeding by pinching or cutting the others at the soil line.
However, on younger sunflower plants, the first true leaves can be severely damaged or completely consumed.
Cutworms can also damage the leaves of young sunflowers, leaving notches or holes. Wilting may also occur.
Once the seeds have sprouted and the first true leaves appear, it's time to transplant the seedlings outside. The pumpkin plants are extremely delicate, and care must be taken when transitioning them outdoors.
Lift the plants from the seed flat when they have developed their first true leaves. Gently pry the plant up as it is being pulled to help prevent breaking the roots. Handle the plant by the leaves, not the stem.
When the first true leaves appear, use sharp scissors to snip some weaker seedlings right at soil level.
When the second set of leaves—the first true leaves—appear, transplant to individual pots or deep containers (such as plastic cups), burying the stems deeper than they stood previously.
Most micro greens are harvested as soon as you see the first true leaves. Because of their size, they're best used as a special touch to a dish.
Plants emerge in 3 to 5 days in adequately warm soil and quickly develop true leaves. When first true leaves develop, thin the plants saving the best three or four plants per hill.
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Definition as written by Windy:
The cotyledon are the embryonic leaves and wither when the first true leaves develop. This is very noticable on a bean seed where sometimes you can see the whole bean split and the cotyledon appear.
To keep the roots from becoming crowded, transplant seedlings into 2 in (5.1 cm) pots as soon as the first true leaves develop, and grow on in a sunny place at 60°F (16°C).
Transplant A seedling that grows its first true leaves, which resemble the adult plant leaves rather than the seed leaves that first appear.
Transplanting The process of digging up a plant and moving it to another location.
It's important to prick out as soon as the plant is ready which is usually when the first true leaves emerge (ie the second set of leaves that emerge after the seed or cotyledon leaves).
The "seed leaves" produced by the embryo of a seed plant that serve to absorb nutrients packaged in the seed, until the seedling is able to produce its first true leaves and begin photosynthesis; ...
Space small seeds 1/8, medium seeds 1/2 and large seeds an inch apart. Space rows 1-1/2 to 2 inches apart to allow seedlings room to grow their first true leaves before transplanting.
After the seedlings have formed their first true leaves, it's time to thin them out. Remove all but the healthiest starts, leaving appropriate spaces between. Follow the recommendations for spacing on the packet of seeds you are using.
Hidden inside that coat is the radicle or beginning of a root, the hypocotyl or beginning of a shoot, either one or two seed leaves or cotyledons (the seed's food supply), and the epicotyl, which will become the first true leaves.
The largest of the seedlings in this image is a tomato (volunteer, it's a lettuce bed, as you can see by the other seedlings). Note how different the first leaves out and the first True leaves are.
Sow two softened seeds one inch deep in individual 4-inch plastic pots filled with sterile soilless mix. Cut out the weaker plant when the seedlings have their first true leaves. Keep them warm, and give them as much light as possible.
When the first true leaves develop, thin to one seedling per container if using peat pots or partitioned trays. If seeds were planted in larger containers, transplant them to smaller containers or peat pots at this stage.
a very small amount of fertilizer, often referred to as a nutrient charge, as well. Most of this will leach out within two weeks after irrigation starts. In fact, many suppliers recommend that liquid fertilization begin as soon the first true leaves ...
Once the first true leaves (the second set to emerge) develop, you can begin to feed your plants. Up until this time the seedlings have been being nourished from the seed itself.
 See also: Plant, True leaves, Seedling, Light, Planting
  
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